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许凌, 余丹丹, 马玉华, 姚玉林, 罗荣华, 冯小丽, 蔡后荣, 韩建保, 王雪卉, 李明华, 柯昌文, 郑永唐, 姚永刚. 2020: SARS-CoV-2感染中国树鼩呈现COVID-19样症状. 动物学研究, 41(5): 517-526. DOI: 10.24272/j.issn.2095-8137.2020.053
引用本文: 许凌, 余丹丹, 马玉华, 姚玉林, 罗荣华, 冯小丽, 蔡后荣, 韩建保, 王雪卉, 李明华, 柯昌文, 郑永唐, 姚永刚. 2020: SARS-CoV-2感染中国树鼩呈现COVID-19样症状. 动物学研究, 41(5): 517-526. DOI: 10.24272/j.issn.2095-8137.2020.053
Ling Xu, Dan-Dan Yu, Yu-Hua Ma, Yu-Lin Yao, Rong-Hua Luo, Xiao-Li Feng, Hou-Rong Cai, Jian-Bao Han, Xue-Hui Wang, Ming-Hua Li, Chang-Wen Ke, Yong-Tang Zheng, Yong-Gang Yao. 2020: COVID-19-like symptoms observed in Chinese tree shrews infected with SARS-CoV-2. Zoological Research, 41(5): 517-526. DOI: 10.24272/j.issn.2095-8137.2020.053
Citation: Ling Xu, Dan-Dan Yu, Yu-Hua Ma, Yu-Lin Yao, Rong-Hua Luo, Xiao-Li Feng, Hou-Rong Cai, Jian-Bao Han, Xue-Hui Wang, Ming-Hua Li, Chang-Wen Ke, Yong-Tang Zheng, Yong-Gang Yao. 2020: COVID-19-like symptoms observed in Chinese tree shrews infected with SARS-CoV-2. Zoological Research, 41(5): 517-526. DOI: 10.24272/j.issn.2095-8137.2020.053

SARS-CoV-2感染中国树鼩呈现COVID-19样症状

COVID-19-like symptoms observed in Chinese tree shrews infected with SARS-CoV-2

  • 摘要: 2019冠状病毒病(COVID-19)大流行对全球公共卫生安全构成了巨大威胁。找到易感染新型冠状病毒(SARS-CoV-2/HCoV-19)的实验动物对于控制疫情、筛选有效的预防或治疗方法都是至关重要的。在本研究中,我们对灵长动物近亲——树鼩进行了新型冠状病毒感染模型创建研究。我们选择了不同年龄段的树鼩,包括1岁左右的成年树鼩和5-6岁的老年树鼩,感染SARS-CoV-2。在树鼩接种病毒后的不同天数进行肺部影像学、病毒载量、血常规和生化以及组织病理学等分析。结果表明,树鼩可以感染SARS-CoV-2病毒。X射线检查可以在大多数感染树鼩看到肺部浸润。在感染后3、5和7天,感染树鼩肺组织中可检测到病毒RNA,血常规和血清生化相关参数也发生变化,包括天冬氨酸转氨酶(AST)和尿素氮(BUN)水平升高。感染后3天成年组和7天老年组树鼩肺组织病理学染色显示肺泡间隔增厚,间质出血。在病毒排出高峰方面,两个不同的年龄组中存在一些差异。我们的研究结果表明,中国树鼩有可能成为COVID-19机制研究、药物和疫苗评价的模型动物。

     

    Abstract: The coronavirus disease 2019 (COVID-19) pandemic continues to pose a global threat to the human population. Identifying animal species susceptible to infection with the SARS-CoV-2/ HCoV-19 pathogen is essential for controlling the outbreak and for testing valid prophylactics or therapeutics based on animal model studies. Here, different aged Chinese tree shrews (adult group, 1 year old; old group, 5–6 years old), which are close relatives to primates, were infected with SARS-CoV-2. X-ray, viral shedding, laboratory, and histological analyses were performed on different days post-inoculation (dpi). Results showed that Chinese tree shrews could be infected by SARS-CoV-2. Lung infiltrates were visible in X-ray radiographs in most infected animals. Viral RNA was consistently detected in lung tissues from infected animals at 3, 5, and 7 dpi, along with alterations in related parameters from routine blood tests and serum biochemistry, including increased levels of aspartate aminotransferase (AST) and blood urea nitrogen (BUN). Histological analysis of lung tissues from animals at 3 dpi (adult group) and 7 dpi (old group) showed thickened alveolar septa and interstitial hemorrhage. Several differences were found between the two different aged groups in regard to viral shedding peak. Our results indicate that Chinese tree shrews have the potential to be used as animal models for SARS-CoV-2 infection.

     

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