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王雪卉, 宋天章, 李磊, 田仁荣, 郑永唐. 2020: 肠道切除吻合术在非人灵长类动物肠道纵向研究中活检取材的可行性研究. 动物学研究, 41(4): 449-454. DOI: 10.24272/j.issn.2095-8137.2020.049
引用本文: 王雪卉, 宋天章, 李磊, 田仁荣, 郑永唐. 2020: 肠道切除吻合术在非人灵长类动物肠道纵向研究中活检取材的可行性研究. 动物学研究, 41(4): 449-454. DOI: 10.24272/j.issn.2095-8137.2020.049
Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian, Yong-Tang Zheng. 2020: Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research. Zoological Research, 41(4): 449-454. DOI: 10.24272/j.issn.2095-8137.2020.049
Citation: Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian, Yong-Tang Zheng. 2020: Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research. Zoological Research, 41(4): 449-454. DOI: 10.24272/j.issn.2095-8137.2020.049

肠道切除吻合术在非人灵长类动物肠道纵向研究中活检取材的可行性研究

Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research

  • 摘要: 肠道活检是研究肠道病理变化的一种基本实验方法。该文详细描述了肠道切除吻合术后对猕猴的肠外营养支持和术后护理,保证成功地对12只猕猴进行了空肠切除吻合术。获得的肠道组织进行苏木精和伊红染色、电子显微镜、流式细胞术、免疫荧光检测和RNA质量分析,证明了活检肠道样本在组织学、生理学、病理学和免疫学检测以及细胞和分子水平方面的适用性。重要的是,手术没有影响猕猴外周血免疫细胞的比例或数量,通过检测血浆中脂类、蛋白质和维生素的浓度也证明,手术没有影响猕猴的营养状况。因此,该文研究结果表明,空肠切除吻合手术是可行的,手术没有影响免疫稳态和肠道屏障的完整性。所有猕猴均恢复良好(除1只外),无术后并发症。肠道切除吻合术可成为AIDS等肠道疾病纵向研究取材的重要活检方法。

     

    Abstract: Intestinal biopsy is a basic experimental method for studying pathological changes in the intestinal tract during human immunodeficiency virus (HIV) infection. In this study, jejunal resection and anastomosis were successfully performed in 12 Chinese rhesus macaques (Macaca mulatta). The sampled gut tissues were then examined by hematoxylin and eosin (H&E) staining, electron microscopy, flow cytometry, immunofluorescence detection, and RNA quality analysis to ensure suitability for histological, physiological, pathological, and immunological detection, as well as mechanistic analysis at the cellular and molecular level. Importantly, the surgery did not affect the ratio or number of immune cells in peripheral blood or the concentration of lipids, proteins, and vitamins in plasma, which are important indicators of nutritional status. Our results thus indicated that jejunal resection and anastomosis are feasible, and that immune homeostasis and intestinal barrier integrity are not altered by surgery. All macaques recovered well (except for one), with no postoperative complications. Therefore, this animal surgery may be applicable for longitudinal intestinal research related to diseases such as acquired immunodeficiency syndrome (AIDS).

     

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