Transcriptomic analyses in humans and mice provide insights into depression
- Received Date: 2020-07-01
- Accepted Date: 2020-09-07
- Rev Recd Date: 2020-08-29
- Available Online: 2020-09-08
Abstract: Accumulating studies have been conducted to identify risk genes and relevant biological mechanisms underlying major depressive disorder (MDD), among which transcriptomic analyses in brain regions engaged in cognitive and emotional processes, e.g., dorsolateral prefrontal cortex (DLPFC), have provided essential insights. Based on three independent DLPFC RNA-seq datasets including 79 MDD patients and 75 healthy controls, we performed differential expression analyses using two alternative approaches for cross-validation. We also conducted transcriptomic analyses in mice underwent chronic variable stress (CVS) and chronic social defeat stress (CSDS) respectively. We identified twelve differentially-expressed genes (DEGs) through both analytical methods in MDD patients, majority of which were also dysregulated in stressed mice. Notably, our analyses identified the immediate early gene FOS (Fos proto-oncogene) whose mRNA level was significantly decreased in both MDD patients as well as mice exposed to CVS, and the mice susceptible to CSDS exhibited a greater reduction of Fos expression compared with resilient mice, suggesting potentially key roles of this gene in the pathogenesis of MDD related to stress exposure. Altered transcriptomes in the DLPFC of MDD patients might (partially) be a result of stress exposure, supporting that stress is a primary risk factor for MDD.
|Citation:||Huijuan Li, Xi Su, Luwen Zhang, Chuyi Zhang, Lu Wang, Wenqiang Li, Yongfeng Yang, Luxian Lv, Ming Li, Xiao Xiao. Transcriptomic analyses in humans and mice provide insights into depression. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.174|